Infrared spectroscopy was performed using a Spectrum One FT-IR with ATR attachment (Perkin Elmer). Sample (average mass or volume ± range) was pressed against the ATR entrance lens and a spectrum acquired from 4000 – 600 cm-1 [modify based on spectral range acquired]. FT-IR experiments and analyses were performed using the Spectrum software from Perkin Elmer.

DLS was performed using a Zetasizer nanoNS (Malvern Pananalytical). Sample suspension was prepared at 1 mg/mL (modify for specific concentration, if different from standard) in HPLC grade water (include any salt or surfactant additives; modify for specific solvent used, if organic solvent used) (Fisher) followed by 0.4 µm filtration. Suspension was measured in a PMMA cuvette (modify for specific cuvette used) and light scattering measurements taken over (range) cm-1 at temperature (range) C for (time) min. DLS experiments and analyses were performed using the Zetasizer software from Malvern.

Thermal analysis was performed using a Discovery 5500 TGA (TA Instruments, New Castle, DE). Sample was dried under dynamic vacuum for 24 h and stored in a desiccator prior to analysis [omit if this step was not performed prior to analysis]. Dried sample (average mass or volume ± range) was tared on a [aluminum, platinum, ceramic] pan (TA Instruments) and measured against a reference pan of the same material. Measurements were performed in nitrogen [modify background gas, if gas was changed from standard] from 25 to [maximum temperature] °C at [ramp rate] °C/min. [insert short method description here, including multiple temperature ramps and/or rates, isothermal events, or gas switching events] TGA experiments and analyses were performed using the TRIOS (v.5.1.1) software from TA Instruments.

Thermal analysis was performed using a Discovery 5500 TGA (TA Instruments, New Castle, DE) with inline Cirrus 3 residual gas analyzer mass spectrometer (MKS). Sample was dried under dynamic vacuum for 24 h and stored in a desiccator prior to analysis [omit if this step was not performed prior to analysis]. Dried sample (average mass or volume ± range) was tared on a [aluminum, platinum, ceramic] pan (TA Instruments) and measured against a reference pan of the same material. After 5 minutes equilibration time at 30 C to pump out excess air during sample introduction, decomposition was performed in nitrogen [modify background gas, if gas was changed from standard] to [maximum temperature] °C at [ramp rate] °C/min. [insert short method description here, including multiple temperature ramps and/or rates, isothermal events, or gas switching events]. Evolved gas was ionized by electron impact (EI) at 75 eV [or 45 eV, if low energy setting was used] and [description of mass scanning experiment: ‘ion mass scanning was performed from 1 – 300 amu [modify based on mass scan range]’; or selected ion monitoring experiment: ‘ions [give ions] amu were selectively monitored’]. TGA experiments and analyses were performed using the TRIOS (v.5.1.1) software from TA Instruments. Mass spectrometry experiments were performed using Process Eye (v.111) software (MKS), individual mass scans were analyzed in Recall (v.111) (MKS), and select ions were analyzed in TRIOS alongside the TGA decomposition.

Thermal analysis was performed using a Discovery 2500 DSC (TA Instruments, New Castle, DE) with a RSC 90 two-stage refrigerated cooling system. Sample (average mass or volume ± range) was sealed in aluminum [standard or hermetic] pans (DSC Consumables) and loaded into the instrument at 40 °C [modify loading temperature, if temperature was changed from standard] with a reference pan of the same material. Under a constant flow of N₂ gas (50 mL/min), the samples underwent [insert short description of your method; eg, three heat-cool-heat cycles between [range] °C with [time] minute isothermals between extremes.] DSC experiments and analyses were performed using the TRIOS (v.5.1.1) software from TA Instruments.

Rheological analysis was performed using a Discovery HR-2 rheometer (TA Instruments, New Castle, DE) using [insert geometry type, e.g., 20 mm cone, 20 mm peltier plate)] geometry. 

Rheology experiments and analyses were performed using the TRIOS (v.5.1.1) software from TA Instruments.

Rheology was performed using a Discovery 850 DMA (TA Instruments, New Castle, DE) with a cooling unit type cooling system. Sample (average length and width ± range) was clamped in clamping geometry type. Samples underwent [insert short description of your method; eg, frequency [range] Hz over temperature [range] °C for [time] min.] DMA experiments and analyses were performed using the TRIOS (v.5.1.1) software from TA Instruments.

Mechanical testing was performed using a F-310 mechanical analyzer (Mark-10). Sample (average length and width ± range) was clamped in pneumatic grips under a constant 1 kN load. Samples underwent [insert short description of your method; eg, elongation testing until failure, 3, 5 minute cycles of elongation and compression testing.] Mechanical testing were performed using the program name software from Mark-10.

Gel permeation chromatography (GPC) was performed using an HP GPC system (Tosoh), including isocratic pump, autosampler, column compartment, viscometer, UV absorbance detector, and internal differential refractive index detector, with an inline MALS detector (Tosoh) [omit detectors not used or indicate specific detectors monitored]. Separation was performed on two Tosoh GMH-HRR GPC columns (7.8 × 300 mm, 5 µm) in series, with preceding guard column of the same material (4.6 × 30 mm, 7 µm). Experiments were conducted with 100 uL injection volumes at 40 °C using HPLC grade tetrahydrofuran (THF) (Fisher) as the mobile phase at a flow rate of 1.0 mL/min. Column calibration was performed with narrow-dispersity polystyrene (PS) standards (Agilent). Samples were prepared at 2 mg/mL [modify concentration, if changed from standard] in HPLC grade THF and left for 24 hours to diffuse into solution followed by 0.22 µm filtration.

Gel permeation chromatography (GPC) was performed using an Agilent Technologies 1260 Infinity series liquid chromatography system, including isocratic pump, autosampler, column compartment, and internal differential refractive index detector. Separation was performed on two Agilent PL-HFIP gel GPC columns (4.6 × 250 mm, 9 µm) in series, with preceding guard column of the same material (4.6 × 50 mm, 9 µm). Experiments were conducted with 100 uL injection volumes at 40 °C using a 20 mM solution of sodium trifluoroacetate (TFA) (Fisher) dissolved in HPLC grade hexafluoroisopropanol (HFIP) (Fisher) as the mobile phase at a flow rate of 0.30 mL/min. Column calibration was performed with narrow-dispersity poly(methyl methacrylate) (PMMA) standards (Polymer Calibration Services). Samples were prepared at 2 mg/mL [modify concentration, if changed from standard] in HFIP mobile phase and left for 24 hours to diffuse into solution followed by 0.22 µm filtration.

Gel permeation chromatography (GPC) was performed using an Agilent Technologies 1260 Infinity series liquid chromatography system, including isocratic pump, autosampler, column compartment, and internal differential refractive index detector. Separation was performed on two Waters Styragel HR-4E GPC columns (7.8 × 300 mm, 5 µm) in series, with preceding guard column of the same material (4.6 × 30 mm, 20 µm). Experiments were conducted with 100 uL injection volumes at 40 °C using 5 mM solution of lithium bromide dissolved in HPLC grade dimethylformamide (DMF) (Fisher) as the mobile phase at a flow rate of 1.0 mL/min. Column calibration was performed with narrow-dispersity polystyrene (PS) standards (Agilent). Samples were prepared at 2 mg/mL [modify concentration, if changed from standard] in DMF mobile phase and left for 24 hours to diffuse into solution followed by 0.22 µm filtration.